研究绘制肝癌中的免疫细胞动态图谱
作者：小柯机器人 发布时间：2019/11/1 10:17:49
北京大学张泽民、任仙文、首都医科大学附属北京世纪坛医院彭吉润以及德国勃林格殷格翰公司刘康等研究人员，合作绘制了肝细胞癌（HCC）中单个免疫细胞的动态图谱。该研究成果于2019年10月31日发表在国际学术期刊《细胞》杂志。
结合两种单细胞RNA测序技术，研究人员在HCC病人中的五个与免疫相关部位获得了CD45+免疫细胞的转录组：肿瘤、邻近肝脏、肝淋巴结（LN）、血液和腹水。LAMP3+树突状细胞（DC）群体似乎是常规DC的成熟形式，具有从肿瘤迁移到LN的潜力。LAMP3+DC还表达了多种免疫相关配体，并具有调节多种淋巴细胞亚型的潜力。在表现出不同转录状态的肿瘤巨噬细胞中，与肿瘤相关的巨噬细胞（TAM）与不良预后相关，研究人员在这些细胞中建立了SLC40A1和GPNMB的炎症作用。此外，腹水中的髓样和淋巴样细胞分别主要与肿瘤和血液起源相关。
这项研究揭示出的各种CD45+细胞类型动态特性为HCC的免疫环境增加了新的维度。
据了解，HCC的免疫微环境尚未完整定义。
附：英文原文
Title:Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma
Author:Qiming Zhang 14,Yao He 14,Nan Luo 14,Shashank J. Patel,Yanjie Han,Ranran Gao,Madhura Modak,Sebastian Carotta,Christian Haslinger,David Kind,Gregory W. Peet,Guojie Zhong,Shuangjia Lu,Weihua Zhu,Yilei Mao
Mengmeng Xiao,Michael Bergmann,Xueda Hu,Sid P. Kerkar,Anne B. Vogt,Stefan Pflanz,Kang Liu,Jirun Peng,Xianwen Ren,Zemin Zhang
Issue&Volume:OCTOBER 31, 2019
Abstract: The immune microenvironment of hepatocellular carcinoma (HCC) is poorly characterized. Combining two single-cell RNA sequencing technologies, we produced transcriptomes of CD45+ immune cells for HCC patients from five immune-relevant sites: tumor, adjacent liver, hepatic lymph node (LN), blood, and ascites. A cluster of LAMP3+ dendritic cells (DCs) appeared to be the mature form of conventional DCs and possessed the potential to migrate from tumors to LNs. LAMP3+ DCs also expressed diverse immune-relevant ligands and exhibited potential to regulate multiple subtypes of lymphocytes. Of the macrophages in tumors that exhibited distinct transcriptional states, tumor-associated macrophages (TAMs) were associated with poor prognosis, and we established the inflammatory role of SLC40A1 and GPNMB in these cells. Further, myeloid and lymphoid cells in ascites were predominantly linked to tumor and blood origins, respectively. The dynamic properties of diverse CD45+ cell types revealed by this study add new dimensions to the immune landscape of HCC.
DOI:10.1016/j.cell.2019.10.003
期刊信息
Cell：《细胞》，创刊于1974年。隶属于细胞出版社，最新IF：36.216