美国哈佛医学院Sean G. Megason和奥地利科学技术学院Carl-Philipp Heisenberg合作取得最新进展。他们提出粘附代码可确保组织形态发生过程中形成牢固模式。这一研究成果发表在2020年10月2日出版的《科学》杂志上。

通过直接测量三种内源性神经祖细胞的粘附力和偏好，他们为其中细胞间粘附差异介导细胞分选的差异粘附模型提供了证据。不同类别的钙黏着蛋白（N-钙黏着蛋白，钙黏着蛋白11和原钙黏着蛋白19）的细胞类型特异性组合表达导致离体同型偏好和体内模式明显。

此外，不同粘附编码是由声波刺猬蛋白原梯度调节的。他们表明在组织形态发生过程中的强大模式是基于粘附的自组织和形态发生导向模式之间相互作用的结果。

据了解，动物的发育需要在时间和空间上组织特定的细胞类型，而且空间模式必须以稳健的方式形成。在斑马鱼脊髓中，尽管形态发生和生长过程中嘈杂的形态发生信号和大规模细胞重排，神经祖细胞仍会形成定型模式。

附：英文原文

Title: An adhesion code ensures robust pattern formation during tissue morphogenesis

Author: Tony Y.-C. Tsai, Mateusz Sikora, Peng Xia, Tugba Colak-Champollion, Holger Knaut, Carl-Philipp Heisenberg, Sean G. Megason

Issue&Volume: 2020/10/02

Abstract: Animal development entails the organization of specific cell types in space and time, and spatial patterns must form in a robust manner. In the zebrafish spinal cord, neural progenitors form stereotypic patterns despite noisy morphogen signaling and large-scale cellular rearrangements during morphogenesis and growth. By directly measuring adhesion forces and preferences for three types of endogenous neural progenitors, we provide evidence for the differential adhesion model in which differences in intercellular adhesion mediate cell sorting. Cell type–specific combinatorial expression of different classes of cadherins (N-cadherin, cadherin 11, and protocadherin 19) results in homotypic preference ex vivo and patterning robustness in vivo. Furthermore, the differential adhesion code is regulated by the sonic hedgehog morphogen gradient. We propose that robust patterning during tissue morphogenesis results from interplay between adhesion-based self-organization and morphogen-directed patterning.

DOI: 10.1126/science.aba6637

Source: https://science.sciencemag.org/content/370/6512/113