近日，美国哈佛医学院Steven P. Gygi、David P. Nusinow等研究人员合作新增了癌细胞系百科全书（CCLE）数据库中的定量蛋白质组学数据。该研究2020年1月23日发表于国际一流学术期刊《细胞》。

Title: Quantitative Proteomics of the Cancer Cell Line Encyclopedia

Author: David P. Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald, Marian Kalocsay, Judit Jané-Valbuena, Ellen Gelfand, Devin K. Schweppe, Mark Jedrychowski, Javad Golji, Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi A. Garraway, William R. Sellers, Steven P. Gygi

Issue&Volume: 2020/01/23

Abstract: Proteins are essential agents of biological processes. To date, large-scale profilingof cell line collections including the Cancer Cell Line Encyclopedia (CCLE) has focusedprimarily on genetic information whereas deep interrogation of the proteome has remainedout of reach. Here, we expand the CCLE through quantitative profiling of thousandsof proteins by mass spectrometry across 375 cell lines from diverse lineages to revealinformation undiscovered by DNA and RNA methods. We observe unexpected correlationswithin and between pathways that are largely absent from RNA. An analysis of microsatelliteinstable (MSI) cell lines reveals the dysregulation of specific protein complexesassociated with surveillance of mutation and translation. These and other proteincomplexes were associated with sensitivity to knockdown of several different genes.These data in conjunction with the wider CCLE are a broad resource to explore cellularbehavior and facilitate cancer research.

DOI: 10.1016/j.cell.2019.12.023

Source: https://www.cell.com/cell/fulltext/S0092-8674(19)31385-6