单神经元投射的高通量测序揭示嗅觉皮层的空间组织，这一成果由美国冷泉港实验室Dinu F. Albeanu等研究人员合作完成。这一研究成果发表在2022年10月27日出版的国际学术期刊《细胞》上。

研究人员使用基于高通量测序的神经解剖学技术，以单细胞分辨率分析了5309个小鼠嗅球和30433个梨状皮层输出神经元的投射。令人惊讶的是，对这个更大的数据集的统计分析显示，嗅觉皮层的连接是有空间结构的。单个嗅球神经元针对沿着皮层前后轴的特定位置，也投射到相匹配的、功能不同的、皮层外的目标。此外，来自目标梨状区的单个神经元也投射到相同的匹配的梨状区外目标，形成三联回路模式。因此，与其他感觉模式一样，嗅觉信息在处理的早期阶段以一种协调的方式被输送到功能不同的目标。

据悉，在大多数感觉模式中，神经元的连接反映了行为上相关的刺激特征，如空间位置、方向和声音频率。相比之下，在嗅觉皮层中，基于几十个神经元的重建，普遍的观点是连接是随机的。

附：英文原文

Title: High-throughput sequencing of single neuron projections reveals spatial organization in the olfactory cortex

Author: Yushu Chen, Xiaoyin Chen, Batuhan Baserdem, Huiqing Zhan, Yan Li, Martin B. Davis, Justus M. Kebschull, Anthony M. Zador, Alexei A. Koulakov, Dinu F. Albeanu

Issue&Volume: 2022/10/27

Abstract: In most sensory modalities, neuronal connectivity reflects behaviorally relevant stimulusfeatures, such as spatial location, orientation, and sound frequency. By contrast,the prevailing view in the olfactory cortex, based on the reconstruction of dozensof neurons, is that connectivity is random. Here, we used high-throughput sequencing-basedneuroanatomical techniques to analyze the projections of 5,309 mouse olfactory bulband 30,433 piriform cortex output neurons at single-cell resolution. Surprisingly,statistical analysis of this much larger dataset revealed that the olfactory cortexconnectivity is spatially structured. Single olfactory bulb neurons targeting a particularlocation along the anterior-posterior axis of piriform cortex also project to matched,functionally distinct, extra-piriform targets. Moreover, single neurons from the targetedpiriform locus also project to the same matched extra-piriform targets, forming triadiccircuit motifs. Thus, as in other sensory modalities, olfactory information is routedat early stages of processing to functionally diverse targets in a coordinated manner.

DOI: 10.1016/j.cell.2022.09.038

Source: https://www.cell.com/cell/fulltext/S0092-8674(22)01257-0