研究人员报道了下一代绿色和红色电压传感器Ace-mNeon2和VARNAM2，以及它们的反向反应极性变体pAce和pAceR。这些指示器能够在清醒的小鼠中对每个视场中超过50个突发性神经元进行0.4-1千赫兹的电压记录，并在苍蝇中进行约30分钟的连续成像。使用双极性多路复用成像，研究人员发现了大脑状态依赖的新皮层生长抑素表达（SST+），和血管活性肠肽表达（VIP+）的中间神经元之间的拮抗作用，以及具有不同轴突的细胞群对海马场电位的贡献。
通过结合三个相互兼容的指示器，研究人员同时进行了三群成像。这些方法将增强对神经元亚类之间在单脉冲分辨率下的动态相互作用研究。
据了解，基因编码的荧光电压指示器非常适合于揭示目标细胞群之间的毫秒级相互作用。然而，目前的指示器缺乏必要的灵敏度，无法在体内进行多群体成像。
附：英文原文
Title: Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types
Author: Madhuvanthi Kannan, Ganesh Vasan, Simon Haziza, Cheng Huang, Radosaw Chrapkiewicz, Junjie Luo, Jessica A. Cardin, Mark J. Schnitzer, Vincent A. Pieribone
Issue&Volume: 2022-11-04
Abstract: Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current indicators lack the requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green and red voltage sensors, Ace-mNeon2 and VARNAM2, and their reverse response-polarity variants pAce and pAceR. Our indicators enable 0.4- to 1-kilohertz voltage recordings from >50 spiking neurons per field of view in awake mice and ~30-minute continuous imaging in flies. Using dual-polarity multiplexed imaging, we uncovered brain state–dependent antagonism between neocortical somatostatin-expressing (SST+) and vasoactive intestinal peptide–expressing (VIP+) interneurons and contributions to hippocampal field potentials from cell ensembles with distinct axonal projections. By combining three mutually compatible indicators, we performed simultaneous triple-population imaging. These approaches will empower investigations of the dynamic interplay between neuronal subclasses at single-spike resolution.
DOI: abm8797
Source: https://www.science.org/doi/10.1126/science.abm8797