中国医学科学院和北京协和医学院Hui Cheng，Tao Cheng，Bo O. Zhou和Bing Liu共同合作近期取得重要工作进展，他们通过单细胞RNA-seq分析揭示了HSC在人类胎儿骨髓中出现的情况。相关研究工作2022年11月3日在线发表于《细胞—干细胞》杂志上。

研究人员使用单细胞RNA测序来绘制人类胚胎BM和脾脏造血干/祖细胞（HSPC）的转录组图谱，及受孕后10至14周（PCW）的微环境。研究人员进一步证明，能够在成年NOG小鼠中重建长期多谱系造血的功能性HSC直到12个PCW才出现在BM中。相反，14个PCW在脾脏中未检测到功能性HSC。通过比较BM和脾脏之间的niche-HSPC相互作用，研究人员确定了可能参与胎儿HSC迁移和维持的配体受体对。

他们的工作为研究人类胎儿骨髓中HSC定植的机制奠定了基础，并为未来HSC的发展研究提供了宝贵的资源。

据介绍，在胎儿发育期间，人类造血干细胞（HSC）定植于骨髓（BM），在那里它们自我更新并维持造血的功能。目前尚不清楚HSC在BM中的播种情况

附：英文原文

Title: Uncovering the emergence of HSCs in the human fetal bone marrow by single-cell RNA-seq analysis

Author: Zhaofeng Zheng, Han He, Xinyu Thomas Tang, Han Zhang, Fanglin Gou, Hua Yang, Jiaxuan Cao, Shujuan Shi, Zining Yang, Guohuan Sun, Xiaowei Xie, Yang Zeng, Aiqing Wen, Yu Lan, Jiaxi Zhou, Bing Liu, Bo O. Zhou, Tao Cheng, Hui Cheng

Issue&Volume: 2022/11/03

Abstract: During fetal development, human hematopoietic stem cells (HSCs) colonize the bonemarrow (BM), where they self-renew and sustain hematopoiesis throughout life; however,the precise timepoint at which HSCs seed the BM is unclear. We used single-cell RNA-sequencingto map the transcriptomic landscape of human fetal BM and spleen hematopoietic stem/progenitorcells (HSPCs) and their microenvironment from 10 to 14 post-conception weeks (PCWs).We further demonstrated that functional HSCs capable of reconstituting long-term multi-lineagehematopoiesis in adult NOG mice do not emerge in the BM until 12 PCWs. In contrast,functional HSCs were not detected in the spleen by 14 PCWs. By comparing the niche-HSPCinteractions between BM and spleen, we identified ligand-receptor pairs likely tobe involved in fetal HSC migration and maintenance. Our work paves the way for researchinto the mechanisms underlying HSC colonization in human fetal BM and provides invaluableresources for future studies on HSC development.

DOI: 10.1016/j.stem.2022.10.005

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(22)00423-4