英国威康桑格研究所Emma L. Rawlins研究小组利用一个人类胎儿肺细胞图谱揭示近端-远端分化梯度和上皮细胞命运的关键调节因子。相关论文发表在2022年12月8日出版的《细胞》杂志上。

研究人员提出了一个人类肺部发育的多组学细胞图谱，其结合了单细胞RNA和ATAC测序、高通量空间转录组学和单细胞成像。将单细胞方法与空间分析相结合，可以对受孕后5-22周的上皮细胞、间质细胞、内皮细胞和红细胞/白细胞区段进行全面的细胞调查。研究人员在所有的区室中都发现了以前未被描述的细胞状态。这些细胞包括发育期特定的分泌祖细胞和与人类小细胞肺癌有关的神经内分泌细胞亚型。

这个数据集可以通过研究人员的网络界面（https://lungcellatlas.org）获得。为了说明其普遍效用，研究人员使用这个细胞图谱来产生关于细胞-细胞信号传导和转录因子层次的预测，并使用类器官模型对其进行了严格的测试。

附：英文原文

Title: A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates

Author: Peng He, Kyungtae Lim, Dawei Sun, Jan Patrick Pett, Quitz Jeng, Krzysztof Polanski, Ziqi Dong, Liam Bolt, Laura Richardson, Lira Mamanova, Monika Dabrowska, Anna Wilbrey-Clark, Elo Madissoon, Zewen Kelvin Tuong, Emma Dann, Chenqu Suo, Isaac Goh, Masahiro Yoshida, Marko Z. Nikoli, Sam M. Janes, Xiaoling He, Roger A. Barker, Sarah A. Teichmann, John C. Marioni, Kerstin B. Meyer, Emma L. Rawlins

Issue&Volume: 2022/12/08

Abstract: We present a multiomic cell atlas of human lung development that combines single-cell RNA and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging. Coupling single-cell methods with spatial analysis has allowed a comprehensive cellular survey of the epithelial, mesenchymal, endothelial, and erythrocyte/leukocyte compartments from 5–22 post-conception weeks. We identify previously uncharacterized cell states in all compartments. These include developmental-specific secretory progenitors and a subtype of neuroendocrine cell related to human small cell lung cancer. Our datasets are available through our web interface (https://lungcellatlas.org). To illustrate its general utility, we use our cell atlas to generate predictions about cell-cell signaling and transcription factor hierarchies which we rigorously test using organoid models.

DOI: 10.1016/j.cell.2022.11.005

Source: https://www.cell.com/cell/fulltext/S0092-8674(22)01415-5