通过结合生物分子马达dynein和DNA结合蛋白，研究人员开发了在DNA纳米管上移动的基于蛋白质的马达。新的马达和基于DNA的纳米架构使研究人员能够在轨道上安排结合点，局部控制运动方向，并通过不同的马达实现多路货物运输。这些技术的整合实现了微尺度的货物分拣器和集成器，可以按照DNA序列的编程在支链DNA纳米管上自动运输分子。这个系统有望为未来的应用提供一个通用的、可控的平台。
据介绍，细胞内运输是细胞内微观物流的基础，由生物分子马达驱动。模仿体外应用的运输已经被广泛研究；然而，轨道设计和控制的不灵活性阻碍了实际应用。
附：英文原文
Title: Programmable molecular transport achieved by engineering protein motors to move on DNA nanotubes
Author: Ryota Ibusuki, Tatsuya Morishita, Akane Furuta, Shintaro Nakayama, Maki Yoshio, Hiroaki Kojima, Kazuhiro Oiwa, Ken’ya Furuta
Issue&Volume: 2022-03-11
Abstract: Intracellular transport is the basis of microscale logistics within cells and is powered by biomolecular motors. Mimicking transport for in vitro applications has been widely studied; however, the inflexibility in track design and control has hindered practical applications. Here, we developed protein-based motors that move on DNA nanotubes by combining a biomolecular motor dynein and DNA binding proteins. The new motors and DNA-based nanoarchitectures enabled us to arrange the binding sites on the track, locally control the direction of movement, and achieve multiplexed cargo transport by different motors. The integration of these technologies realized microscale cargo sorters and integrators that automatically transport molecules as programmed in DNA sequences on a branched DNA nanotube. Our system should provide a versatile, controllable platform for future applications.
DOI: abj5170
Source: https://www.science.org/doi/10.1126/science.abj5170#con8