比利时鲁汶大学Stein Aerts、美国斯坦福大学骆利群、Stephen R. Quake、瑞士洛桑联邦理工学院 (EPFL) Bart Deplancke以及美国哈佛医学院Norbert Perrimon研究团队合作取得进展。他们绘制了果蝇细胞图谱，成年果蝇的单核转录组图谱。2022年3月4日出版的《科学》杂志发表了这项成果。
他们展示了成年果蝇 Tabula Drosophilae 的单细胞图谱，其中包括来自 15 个单独解剖的有性组织以及整个头部和身体的 580,000 个细胞核，注释为 > 250 种不同的细胞类型。他们对整个动物的细胞类型相关基因特征和转录因子标记以及两性二态性进行了深入分析。 对组织间常见细胞类型（如血液和肌肉细胞）的分析揭示了稀有细胞类型和组织特异性亚型。 该图集为果蝇群落提供了宝贵的资源，并可作为研究单细胞分辨率下的遗传扰动和疾病模型的参考。
据悉，100 多年来，果蝇一直是研究最多的模式生物之一。
附：英文原文
Title: Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly
Author: Hongjie Li, Jasper Janssens, Maxime De Waegeneer, Sai Saroja Kolluru, Kristofer Davie, Vincent Gardeux, Wouter Saelens, Fabrice P. A. David, Maria Brbi, Katina Spanier, Jure Leskovec, Colleen N. McLaughlin, Qijing Xie, Robert C. Jones, Katja Brueckner, Jiwon Shim, Sudhir Gopal Tattikota, Frank Schnorrer, Katja Rust, Todd G. Nystul, Zita Carvalho-Santos, Carlos Ribeiro, Soumitra Pal, Sharvani Mahadevaraju, Teresa M. Przytycka, Aaron M. Allen, Stephen F. Goodwin, Cameron W. Berry, Margaret T. Fuller, Helen White-Cooper, Erika L. Matunis, Stephen DiNardo, Anthony Galenza, Lucy Erin O’Brien, Julian A. T. Dow, FCA Consortium§, Heinrich Jasper, Brian Oliver, Norbert Perrimon, Bart Deplancke, Stephen R. Quake, Liqun Luo, Stein Aerts, Devika Agarwal, Yasir Ahmed-Braimah, Michelle Arbeitman, Majd M. Ariss, Jordan Augsburger, Kumar Ayush, Catherine C. Baker, Torsten Banisch, Katja Birker, Rolf Bodmer, Benjamin Bolival, Susanna E. Brantley, Julie A. Brill, Nora C. Brown, Norene A. Buehner, Xiaoyu Tracy Cai, Rita Cardoso-Figueiredo, Fernando Casares, Amy Chang, Thomas R. Clandinin, Sheela Crasta, Claude Desplan, Angela M. Detweiler, Darshan B. Dhakan, Erika Donà, Stefanie Engert, Swann Floc’hlay, Nancy George, Amanda J. González-Segarra, Andrew K. Groves, Samantha Gumbin, Yanmeng Guo, Devon E. Harris, Yael Heifetz, Stephen L. Holtz, Felix Horns, Bruno Hudry, Ruei-Jiun Hung, Yuh Nung Jan, Jacob S. Jaszczak, Gregory S. X. E. Jefferis, Jim Karkanias, Timothy L. Karr, Nadja Sandra Katheder, James Kezos, Anna A. Kim, Seung K. Kim, Lutz Kockel, Nikolaos Konstantinides, Thomas B. Kornberg, Henry M. Krause, Andrew Thomas Labott, Meghan Laturney, Ruth Lehmann, Sarah Leinwand, Jiefu Li, Joshua Shing Shun Li, Kai Li, Ke Li, Liying Li, Tun Li, Maria Litovchenko, Han-Hsuan Liu, Yifang Liu, Tzu-Chiao Lu, Jonathan Manning, Anjeli Mase, Mikaela Matera-Vatnick, Neuza Reis Matias, Caitlin E. McDonough-Goldstein, Aaron McGeever, Alex D. McLachlan, Paola Moreno-Roman, Norma Neff, Megan Neville, Sang Ngo, Tanja Nielsen, Caitlin E. O’Brien, David Osumi-Sutherland, Mehmet Neset zel, Irene Papatheodorou, Maja Petkovic, Clare Pilgrim, Angela Oliveira Pisco, Carolina Reisenman, Erin Nicole Sanders, Gilberto dos Santos, Kristin Scott, Aparna Sherlekar, Philip Shiu, David Sims, Rene V. Sit, Maija Slaidina, Harold E. Smith, Gabriella Sterne, Yu-Han Su, Daniel Sutton, Marco Tamayo, Michelle Tan, Ibrahim Tastekin, Christoph Treiber, David Vacek, Georg Vogler, Scott Waddell, Wanpeng Wang, Rachel I. Wilson, Mariana F. Wolfner
Issue&Volume: 2022-03-04
Abstract: For more than 100 years, the fruit fly Drosophila melanogaster has been one of the most studied model organisms. Here, we present a single-cell atlas of the adult fly, Tabula Drosophilae, that includes 580,000 nuclei from 15 individually dissected sexed tissues as well as the entire head and body, annotated to >250 distinct cell types. We provide an in-depth analysis of cell type–related gene signatures and transcription factor markers, as well as sexual dimorphism, across the whole animal. Analysis of common cell types between tissues, such as blood and muscle cells, reveals rare cell types and tissue-specific subtypes. This atlas provides a valuable resource for the Drosophila community and serves as a reference to study genetic perturbations and disease models at single-cell resolution.
DOI: abk2432
Source: https://www.science.org/doi/10.1126/science.abk2432