瑞士洛桑联邦理工学院Giovanni D’Angelo和Gioele La Manno小组合作取得一项新成果。经过不懈努力，他们发现鞘脂调控真皮成纤维细胞的异质性。2022年4月15日出版的《科学》发表了这项成果。

研究人员通过将高分辨率质谱成像与单细胞转录组学相结合的方法，测量了单个人真皮成纤维细胞的脂质组和转录组。研究发现特定脂质代谢途径的细胞间变化有助于与皮肤结构组织相关细胞状态的建立。鞘脂成分可区分不同成纤维细胞亚群，鞘脂代谢重塑诱导了细胞状态转换。因此，细胞间脂质异质性影响细胞状态的建立，揭示了一种新的多细胞系统自组织调节成分。

据了解，人体细胞会产生数千种脂质，这些脂质在细胞分化过程中会发生变化，并且这些变化可在同一类型的单个细胞中发生。 然而，对于细胞间脂质组成差异的功能研究才刚刚开始。

附：英文原文

Title: Sphingolipids control dermal fibroblast heterogeneity

Author: Laura Capolupo, Irina Khven, Alex R. Lederer, Luigi Mazzeo, Galina Glousker, Sylvia Ho, Francesco Russo, Jonathan Paz Montoya, Dhaka R. Bhandari, Andrew P. Bowman, Shane R. Ellis, Romain Guiet, Olivier Burri, Johanna Detzner, Johannes Muthing, Krisztian Homicsko, Franois Kuonen, Michel Gilliet, Bernhard Spengler, Ron M. A. Heeren, G. Paolo Dotto, Gioele La Manno, Giovanni D’Angelo

Issue&Volume: 2022-04-15

Abstract: Human cells produce thousands of lipids that change during cell differentiation and can vary across individual cells of the same type. However, we are only starting to characterize the function of these cell-to-cell differences in lipid composition. Here, we measured the lipidomes and transcriptomes of individual human dermal fibroblasts by coupling high-resolution mass spectrometry imaging with single-cell transcriptomics. We found that the cell-to-cell variations of specific lipid metabolic pathways contribute to the establishment of cell states involved in the organization of skin architecture. Sphingolipid composition is shown to define fibroblast subpopulations, with sphingolipid metabolic rewiring driving cell-state transitions. Therefore, cell-to-cell lipid heterogeneity affects the determination of cell states, adding a new regulatory component to the self-organization of multicellular systems.

DOI: abh1623

Source: https://www.science.org/doi/10.1126/science.abh1623