研究人员创建了一个人类参考图集，包括来自24个不同组织和器官的近50万个细胞，其中许多来自同一个捐赠者。这个图集使400多种细胞类型的分子特征、它们在组织间的分布以及组织特有的基因表达变化成为可能。使用来自一个捐赠者的多个组织，研究人员能够识别组织间T细胞的克隆分布，识别B细胞的组织特异性突变率，并分析细胞周期状态和跨组织共享细胞类型的增殖潜力。研究人员发现了细胞类型特异性的RNA剪接，并分析了个体内各组织的情况。
据悉，使用单细胞转录组测序的细胞类型的分子特征正在彻底改变细胞生物学，并使人们对人类器官的生理学有了新的认识。
附：英文原文
Title: The Tabula Sapiens: A multiple-organ, single-cell transcriptomic atlas of humans
Author: The Tabula Sapiens Consortium*, Robert C. Jones, Jim Karkanias, Mark A. Krasnow, Angela Oliveira Pisco, Stephen R. Quake, Julia Salzman, Nir Yosef, Bryan Bulthaup, Phillip Brown, William Harper, Marisa Hemenez, Ravikumar Ponnusamy, Ahmad Salehi, Bhavani A. Sanagavarapu, Eileen Spallino, Ksenia A. Aaron, Waldo Concepcion, James M. Gardner, Burnett Kelly, Nikole Neidlinger, Zifa Wang, Sheela Crasta, Saroja Kolluru, Maurizio Morri, Angela Oliveira Pisco, Serena Y. Tan, Kyle J. Travaglini, Chenling Xu, Marcela Alcántara-Hernández, Nicole Almanzar, Jane Antony, Benjamin Beyersdorf, Deviana Burhan, Kruti Calcuttawala, Matthew M. Carter, Charles K. F. Chan, Charles A. Chang, Stephen Chang, Alex Colville, Sheela Crasta, Rebecca N. Culver, Ivana Cvijovi, Gaetano D’Amato, Camille Ezran, Francisco X. Galdos, Astrid Gillich, William R. Goodyer, Yan Hang, Alyssa Hayashi, Sahar Houshdaran, Xianxi Huang, Juan C. Irwin, SoRi Jang, Julia Vallve Juanico, Aaron M. Kershner, Soochi Kim, Bernhard Kiss, Saroja Kolluru, William Kong, Maya E. Kumar, Angera H. Kuo, Rebecca Leylek, Baoxiang Li, Gabriel B. Loeb, Wan-Jin Lu, Sruthi Mantri, Maxim Markovic, Patrick L. McAlpine, Antoine de Morree, Maurizio Morri, Karim Mrouj, Shravani Mukherjee, Tyler Muser, Patrick Neuhfer, Thi D. Nguyen, Kimberly Perez, Ragini Phansalkar, Angela Oliveira Pisco, Nazan Puluca, Zhen Qi, Poorvi Rao, Hayley Raquer-McKay, Nicholas Schaum, Bronwyn Scott, Bobak Seddighzadeh, Joe Segal, Sushmita Sen, Shaheen Sikandar, Sean P. Spencer, Lea C. Steffes, Varun R. Subramaniam, Aditi Swarup, Michael Swift, Kyle J. Travaglini, Will Van Treuren, Emily Trimm, Stefan Veizades, Sivakamasundari Vijayakumar, Kim Chi Vo, Sevahn K. Vorperian, Wanxin Wang, Hannah N. W. Weinstein, Juliane Winkler, Timothy T. H. Wu, Jamie Xie, Andrea R. Yung, Yue Zhang, Angela M. Detweiler, Honey Mekonen, Norma F. Neff, Rene V. Sit, Michelle Tan, Jia Yan, Gregory R. Bean, Vivek Charu, Erna Forgó, Brock A. Martin, Michael G. Ozawa, Oscar Silva, Serena Y. Tan, Angus Toland, Venkata N. P. Vemuri, Shaked Afik, Kyle Awayan, Olga Borisovna Botvinnik, Ashley Byrne, Michelle Chen, Roozbeh Dehghannasiri, Angela M. Detweiler, Adam Gayoso, Alejandro A. Granados, Qiqing Li, Gita Mahmoudabadi, Aaron McGeever, Antoine de Morree, Julia Eve Olivieri, Madeline Park, Angela Oliveira Pisco, Neha Ravikumar, Julia Salzman, Geoff Stanley, Michael Swift, Michelle Tan, Weilun Tan, Alexander J. Tarashansky, Rohan Vanheusden, Sevahn K. Vorperian, Peter Wang, Sheng Wang
Issue&Volume: 2022-05-13
Abstract: Molecular characterization of cell types using single-cell transcriptome sequencing is revolutionizing cell biology and enabling new insights into the physiology of human organs. We created a human reference atlas comprising nearly 500,000 cells from 24 different tissues and organs, many from the same donor. This atlas enabled molecular characterization of more than 400 cell types, their distribution across tissues, and tissue-specific variation in gene expression. Using multiple tissues from a single donor enabled identification of the clonal distribution of T cells between tissues, identification of the tissue-specific mutation rate in B cells, and analysis of the cell cycle state and proliferative potential of shared cell types across tissues. Cell type–specific RNA splicing was discovered and analyzed across tissues within an individual.
DOI: abl4896
Source: https://www.science.org/doi/10.1126/science.abl4896