在本研究中，研究人员使用多重防错荧光原位杂交技术 (MERFISH) 对4000个基因进行了空间分辨的单细胞分析，鉴定了100多个转录不同的细胞群，并生成了人类颞中颞上脑区的分子定义和空间分辨细胞图谱。研究人员进一步探索了以细胞类型特异性方式由体细胞接触或接近引起的细胞间相互作用。人类和小鼠皮质的比较显示细胞层状组织的保守性和跨物种体细胞相互作用的差异。 他们的数据揭示了人类特定的细胞-细胞接近模式以及人类皮层中神经元和非神经元细胞之间相互作用的显著增加。
据介绍，人类的大脑皮层具有巨大的细胞多样性。人类皮层中不同类型的细胞是如何组织的，以及不同物种的细胞组织是如何变化的目前仍不清楚。
附：英文原文
Title: Conservation and divergence of cortical cell organization in human and mouse revealed by MERFISH
Author: Rongxin Fang, Chenglong Xia, Jennie L. Close, Meng Zhang, Jiang He, Zhengkai Huang, Aaron R. Halpern, Brian Long, Jeremy A. Miller, Ed S. Lein, Xiaowei Zhuang
Issue&Volume: 2022-07-01
Abstract: The human cerebral cortex has tremendous cellular diversity. How different cell types are organized in the human cortex and how cellular organization varies across species remain unclear. In this study, we performed spatially resolved single-cell profiling of 4000 genes using multiplexed error-robust fluorescence in situ hybridization (MERFISH), identified more than 100 transcriptionally distinct cell populations, and generated a molecularly defined and spatially resolved cell atlas of the human middle and superior temporal gyrus. We further explored cell-cell interactions arising from soma contact or proximity in a cell type–specific manner. Comparison of the human and mouse cortices showed conservation in the laminar organization of cells and differences in somatic interactions across species. Our data revealed human-specific cell-cell proximity patterns and a markedly increased enrichment for interactions between neurons and non-neuronal cells in the human cortex.
DOI: abm1741
Source: https://www.science.org/doi/10.1126/science.abm1741