近日，
研究人员进行了空间增强分辨率全向测序（Stereo-seq），以捕获蝾螈端脑部分在发育和再生期间的空间分辨率单细胞转录组。标注的细胞类型表现出不同的空间分布、分子特征和功能。研究人员在伤口处发现了一个损伤诱导的上皮细胞群，通过一个类似于发育过程中神经发生的细胞状态转变过程，作为潜在补充失去神经元的祖细胞群。转录组的比较表明，这些诱导的细胞可能来源于局部的常驻上皮细胞。研究人员进一步在病变部位发现了空间上确定的神经元，这些神经元可能退行到类似于未成熟神经元的状态。
这项工作建立了四足动物大脑的空间转录组图谱，并对发育和再生的上皮细胞的潜在神经发生进行了解码，从而为脊椎动物的大脑再生提供了机制性见解。
据悉，脊椎动物的大脑再生的分子机制仍然难以捉摸。
附：英文原文
Title: Single-cell Stereo-seq reveals induced progenitor cells involved in axolotl brain regeneration
Author: Xiaoyu Wei, Sulei Fu, Hanbo Li, Yang Liu, Shuai Wang, Weimin Feng, Yunzhi Yang, Xiawei Liu, Yan-Yun Zeng, Mengnan Cheng, Yiwei Lai, Xiaojie Qiu, Liang Wu, Nannan Zhang, Yujia Jiang, Jiangshan Xu, Xiaoshan Su, Cheng Peng, Lei Han, Wilson Pak-Kin Lou, Chuanyu Liu, Yue Yuan, Kailong Ma, Tao Yang, Xiangyu Pan, Shang Gao, Ao Chen, Miguel A. Esteban, Huanming Yang, Jian Wang, Guangyi Fan, Longqi Liu, Liang Chen, Xun Xu, Ji-Feng Fei, Ying Gu
Issue&Volume: 2022-09-02
Abstract: The molecular mechanism underlying brain regeneration in vertebrates remains elusive. We performed spatial enhanced resolution omics sequencing (Stereo-seq) to capture spatially resolved single-cell transcriptomes of axolotl telencephalon sections during development and regeneration. Annotated cell types exhibited distinct spatial distribution, molecular features, and functions. We identified an injury-induced ependymoglial cell cluster at the wound site as a progenitor cell population for the potential replenishment of lost neurons, through a cell state transition process resembling neurogenesis during development. Transcriptome comparisons indicated that these induced cells may originate from local resident ependymoglial cells. We further uncovered spatially defined neurons at the lesion site that may regress to an immature neuron–like state. Our work establishes spatial transcriptome profiles of an anamniote tetrapod brain and decodes potential neurogenesis from ependymoglial cells for development and regeneration, thus providing mechanistic insights into vertebrate brain regeneration.
DOI: abp9444
Source: https://www.science.org/doi/10.1126/science.abp9444