丹麦奥尔胡斯大学
研究人员表示，了解多组分受体复合物的组成和活化是生物学中的一个挑战。
附：英文原文
Title: Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis
Author: Henriette Rübsam, Christina Krnauer, Nikolaj B. Abel, Hongtao Ji, Damiano Lironi, Simon B. Hansen, Marcin Nadzieja, Marie V. Kolte, Drte Abel, Noor de Jong, Lene H. Madsen, Huijun Liu, Jens Stougaard, Simona Radutoiu, Kasper R. Andersen
Issue&Volume: 2023-01-20
Abstract: Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
DOI: ade9204
Source: https://www.science.org/doi/10.1126/science.ade9204