美国索尔克生物研究所Satchidananda Panda团队近期取得重要工作进展，他们研究绘制了哺乳动物多组织对时间限制性喂养的昼夜转录组图谱。相关研究成果2023年1月3日在线发表于《细胞—代谢》杂志上。

据介绍，时间限制性喂养（TRF）是一种新兴的行为营养干预，涉及每天的喂养和禁食周期。在动物和人类中，TRF具有多器官系统产生的多效性健康益处，但TRF介导益处的分子基础尚不清楚。

研究人员让小鼠接受等热量自由进食（ALF）或西方饮食的TRF，并在24小时内每2小时从22个器官和大脑区域采集的样本中检测基因表达的变化。研究人员发现TRF深刻影响基因表达。在至少一个组织中，近80%的基因在TRF下表现出差异表达或节律性。这些变化的功能注释揭示了TRF的组织和通路特异性影响。

总之，这些发现和资源为未来的机制研究提供了关键基础，并将有助于指导人类限时进食（TRE）干预措施，以治疗各种疾病，无论是否使用药物治疗。

附：英文原文

Title: Diurnal transcriptome landscape of a multi-tissue response to time-restricted feeding in mammals

Author: Shaunak Deota, Terry Lin, Amandine Chaix, April Williams, Hiep Le, Hugo Calligaro, Ramesh Ramasamy, Ling Huang, Satchidananda Panda

Issue&Volume: 2023/01/03

Abstract: Time-restricted feeding (TRF) is an emerging behavioral nutrition intervention that involves a daily cycle of feeding and fasting. In both animals and humans, TRF has pleiotropic health benefits that arise from multiple organ systems, yet the molecular basis of TRF-mediated benefits is not well understood. Here, we subjected mice to isocaloric ad libitum feeding (ALF) or TRF of a western diet and examined gene expression changes in samples taken from 22 organs and brain regions collected every 2 h over a 24-h period. We discovered that TRF profoundly impacts gene expression. Nearly 80% of all genes show differential expression or rhythmicity under TRF in at least one tissue. Functional annotation of these changes revealed tissue- and pathway-specific impacts of TRF. These findings and resources provide a critical foundation for future mechanistic studies and will help to guide human time-restricted eating (TRE) interventions to treat various disease conditions with or without pharmacotherapies.

DOI: 10.1016/j.cmet.2022.12.006

Source: https://www.cell.com/cell-metabolism/fulltext/S1550-4131(22)00543-5