研究人员描述了鹿角再生的单细胞图谱。最早阶段的鹿角启动细胞是表达配对相关同源盒1基因的间充质细胞（PRRX1+间充质细胞）。研究人员还鉴定了一个由PRRX1+间充质细胞发育而来的“鹿角母细胞祖细胞”（ABPC）群体，并指导鹿角再生过程。跨物种比较在几种哺乳动物胚细胞中发现了ABPC。它在体内和体外均表现出较强的自我更新能力，并能生成骨软骨谱系细胞。最后，研究人员观察到鹿角生长中心在生长高峰阶段的细胞和基因表达在空间上结构良好，这揭示了鹿角快速伸长的细胞机制。
据悉，鹿角的每年再生为研究哺乳动物器官再生提供了有价值的模型。
附：英文原文
Title: A population of stem cells with strong regenerative potential discovered in deer antlers
Author: Tao Qin, Guokun Zhang, Yi Zheng, Shengyou Li, Yuan Yuan, Qingjie Li, Mingliang Hu, Huazhe Si, Guanning Wei, Xueli Gao, Xinxin Cui, Bing Xia, Jing Ren, Kun Wang, Hengxing Ba, Zhen Liu, Rasmus Heller, Zhipeng Li, Wen Wang, Jinghui Huang, Chunyi Li, Qiang Qiu
Issue&Volume: 2023-02-24
Abstract: The annual regrowth of deer antlers provides a valuable model for studying organ regeneration in mammals. We describe a single-cell atlas of antler regrowth. The earliest-stage antler initiators were mesenchymal cells that express the paired related homeobox 1 gene (PRRX1+ mesenchymal cells). We also identified a population of “antler blastema progenitor cells” (ABPCs) that developed from the PRRX1+ mesenchymal cells and directed the antler regeneration process. Cross-species comparisons identified ABPCs in several mammalian blastema. In vivo and in vitro ABPCs displayed strong self-renewal ability and could generate osteochondral lineage cells. Last, we observed a spatially well-structured pattern of cellular and gene expression in antler growth center during the peak growth stage, revealing the cellular mechanisms involved in rapid antler elongation.
DOI: add0488
Source: https://www.science.org/doi/10.1126/science.add0488