该研究团队利用最近引入的超分辨技术MINFLUX，开发了一种活细胞内单个荧光团追踪的方法，能够以纳米空间和毫秒时间分辨率在二维和三维中实现对分子机器动态特性的测量。研究团队利用这种方法成功解析了活细胞中马达蛋白kinesin-1在微管上的精确步进运动，并且通过对在固定细胞微管上行走的马达蛋白进行纳米级追踪，实现了以原纤维分辨率解析微管细胞骨架的结构。
据介绍，动态测量分子机器的特性可以为人类了解其机制提供极其重要的洞见，但该测量在活细胞中进行一直具有挑战性。
附：英文原文
Title: Direct observation of motor protein stepping in living cells using MINFLUX
Author: Takahiro Deguchi, Malina K. Iwanski, Eva-Maria Schentarra, Christopher Heidebrecht, Lisa Schmidt, Jennifer Heck, Tobias Weihs, Sebastian Schnorrenberg, Philipp Hoess, Sheng Liu, Veronika Chevyreva, Kyung-Min Noh, Lukas C. Kapitein, Jonas Ries
Issue&Volume: 2023-03-10
Abstract: Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dimensions using the recently introduced super-resolution technique MINFLUX. Using this approach, we resolved the precise stepping motion of the motor protein kinesin-1 as it walked on microtubules in living cells. Nanoscopic tracking of motors walking on the microtubules of fixed cells also enabled us to resolve the architecture of the microtubule cytoskeleton with protofilament resolution.
DOI: ade2676
Source: https://www.science.org/doi/10.1126/science.ade2676