近日，昆明理工大学谭韬等研究人员合作实现从囊胚到早期器官发生的子宫外猴子胚胎发育。这一研究成果于2023年5月11日发表在国际学术期刊《细胞》上。

研究人员表示，灵长类动物妊娠期的第三和第四周有几个发育的里程碑，包括原肠运动和器官原基的形成。然而，由于对体内胚胎的获取受到限制，人们对这一时期的理解是有限的。

为了解决这个问题，研究人员开发了一个嵌入式三维培养系统，可以将猴胚胎在子宫外培养到受精后25天。形态学、组织学和单细胞RNA测序分析表明，子宫外培养的猴胚胎基本上再现了体内发育的关键事件。通过这个平台，研究人员能够勾勒出猴子的神经诱导、侧板中胚层分化、卵黄囊造血、原始肠道和原始生殖细胞样细胞发育所涉及的系谱轨迹和遗传程序。这个嵌入式三维培养系统为猴子胚胎从囊胚到早期器官发生的生长和研究灵长类动物子宫外的胚胎发育提供了一个强大而可重复的平台。

附：英文原文

Title: Ex utero monkey embryogenesis from blastocyst to early organogenesis

Author: Yandong Gong, Bing Bai, Nianqin Sun, Baiquan Ci, Honglian Shao, Ting Zhang, Hui Yao, Youyue Zhang, Yuyu Niu, Lizhong Liu, Hu Zhao, Hao Wu, Lei Zhang, Tianxiang Wang, Shangang Li, Yulei Wei, Yang Yu, Ana Elisa Ribeiro Orsi, Bing Liu, Weizhi Ji, Jun Wu, Yongchang Chen, Tao Tan

Issue&Volume: 2023/05/11

Abstract: The third and fourth weeks of gestation in primates are marked by several developmental milestones, including gastrulation and the formation of organ primordia. However, our understanding of this period is limited due to restricted access to in vivo embryos. To address this gap, we developed an embedded 3D culture system that allows for the extended ex utero culture of cynomolgus monkey embryos for up to 25 days post-fertilization. Morphological, histological, and single-cell RNA-sequencing analyses demonstrate that ex utero cultured monkey embryos largely recapitulated key events of in vivo development. With this platform, we were able to delineate lineage trajectories and genetic programs involved in neural induction, lateral plate mesoderm differentiation, yolk sac hematopoiesis, primitive gut, and primordial germ-cell-like cell development in monkeys. Our embedded 3D culture system provides a robust and reproducible platform for growing monkey embryos from blastocysts to early organogenesis and studying primate embryogenesis ex utero.

DOI: 10.1016/j.cell.2023.04.020

Source: https://www.cell.com/cell/fulltext/S0092-8674(23)00416-6