清华大学Xiaotong Wu等研究人员合作发现，Znf706调控斑马鱼中生殖质的组装及原始生殖细胞的发育。相关论文于2024年11月20日在线发表于国际学术期刊《遗传学报》。

研究人员发现母源性转录因子Znf706调控了生殖质因子，在原始生殖细胞（PGC）迁移过程中形成的核周颗粒状结构的组装。母源和合子znf706突变体（MZznf706）表现出早期胚胎阶段生殖质散乱、PGC数量减少且迁移过程中部分位置异常，以及成年后雌性比例降低。

值得注意的是，在未成熟卵母细胞中实施Znf706的CUT&Tag和RNA-seq分析表明，Znf706在I期卵母细胞中可能促进一些线粒体基因的转录，此外还具有其他功能。

因此，研究人员提出Znf706参与了斑马鱼的生殖质组装及PGC发育过程。

据了解，斑马鱼中PGC的命运由母源性沉积的生殖质预先决定，这些生殖质在卵母细胞中被包装成核糖核蛋白复合物，并遗传到胚胎中命运决定为PGC的细胞。然而，调控生殖质组装和PGC发育的母源性因子尚不清楚。

附：英文原文

Title: Znf706 regulates germ plasm assembly and primordial germ cell development in zebrafish

Author: Anming Meng a e, Xiaotong Wu a

Issue&Volume: 2024/11/20

Abstract: The cell fate of primordial germ cells (PGCs) in zebrafish is pre-determined by maternally deposited germ plasm, which is packaged into ribonucleoprotein complex in oocytes and inherited into PGCs-fated cells in embryos. However, the maternal factors regulating the assembly of germ plasm and PGC development remain poorly understood. In this study, we report that the maternal transcription factor Znf706 regulates the assembly of germ plasm factors into a granule-like structure localized perinuclearly in PGCs during migration. Maternal and zygotic mutants of znf706 (MZznf706) exhibit deficient germ plasm scattering at the early embryonic stage, decreased PGC numbers with some mislocation during PGC migration, and a lower female ratio in adulthood. Notably, the implementation of Znf706 CUT&Tag and RNA-seq on immature oocytes uncovers that Znf706 in stage I oocytes may promote transcription of several mitochondrial genes in addition to other functions. Hence, we propose that Znf706 is implicated in germ plasm assembly and PGC development in zebrafish.

DOI: 10.1016/j.jgg.2024.11.007

Source: https://www.sciencedirect.com/science/article/pii/S1673852724003084