研究人员利用Visium空间基因表达平台生成了横跨人类背外侧前额叶皮层前后轴的，数据驱动型分子神经解剖图谱。与匹配单核RNA测序数据的整合，揭示了跨空间域的独特细胞类型组成和细胞间的相互作用。利用PsychENCODE和公开可用的数据，研究人员将与神经精神疾病相关的细胞类型和基因的富集映射到离散的空间域。
研究人员表示，人类新皮层的分子组织历来是在组织学层的背景下进行研究的。然而，新兴的空间转录组学技术能够无偏倚地识别转录定义的空间域，从而超越经典的细胞结构。
附：英文原文
Title: A data-driven single-cell and spatial transcriptomic map of the human prefrontal cortex
Author: Louise A. Huuki-Myers, Abby Spangler, Nicholas J. Eagles, Kelsey D. Montgomery, Sang Ho Kwon, Boyi Guo, Melissa Grant-Peters, Heena R. Divecha, Madhavi Tippani, Chaichontat Sriworarat, Annie B. Nguyen, Prashanthi Ravichandran, Matthew N. Tran, Arta Seyedian, PsychENCODE Consortium, Thomas M. Hyde, Joel E. Kleinman, Alexis Battle, Stephanie C. Page, Mina Ryten, Stephanie C. Hicks, Keri Martinowich, Leonardo Collado-Torres, Kristen R. Maynard
Issue&Volume: 2024-05-24
Abstract: The molecular organization of the human neocortex historically has been studied in the context of its histological layers. However, emerging spatial transcriptomic technologies have enabled unbiased identification of transcriptionally defined spatial domains that move beyond classic cytoarchitecture. We used the Visium spatial gene expression platform to generate a data-driven molecular neuroanatomical atlas across the anterior-posterior axis of the human dorsolateral prefrontal cortex. Integration with paired single-nucleus RNA-sequencing data revealed distinct cell type compositions and cell-cell interactions across spatial domains. Using PsychENCODE and publicly available data, we mapped the enrichment of cell types and genes associated with neuropsychiatric disorders to discrete spatial domains.
DOI: adh1938
Source: https://www.science.org/doi/10.1126/science.adh1938