英国帝国理工学院化学工程系Yuval Elani研究组开发出了用于原位生化合成和多阶段释放的纳米级脂质体包脂质体。该研究于2024年7月15日发表于国际一流学术期刊《自然—化学》杂志上。

据介绍，软物质纳米级组件，如脂质体和脂质纳米颗粒，具有以外部刺激和特定部位的方式递送和释放多种货物的潜力。这样的组件目前结构简单，包括球形胶囊或脂质团簇。考虑到形式和功能是交织在一起的，这种结构复杂性的缺乏限制了更复杂属性的开发。

为了解决这个问题，该研究团队设计了一种结合微流体和偶联化学的工程策略，来合成具有两个离散室的纳米级脂质体，一个在另一个内，该研究团队称之为浓缩体。研究人员可以控制每一个双层的组成，调整颗粒大小和内外膜之间的尺寸。

该研究团队可以指定每个隔间内封装货物的成份，以及内外双层的生物物理特征，使它们能够赋予每个双层不同的刺激响应特性。研究组将这些颗粒用于在确定的时间点两个有效载荷的多级释放，并作为一个埃升反应器用于触发原位生化合成。

附：英文原文

Title: Engineering a nanoscale liposome-in-liposome for in situ biochemical synthesis and multi-stage release

Author: Pilkington, Colin P., Gispert, Ignacio, Chui, Suet Y., Seddon, John. M., Elani, Yuval

Issue&Volume: 2024-07-15

Abstract: Soft-matter nanoscale assemblies such as liposomes and lipid nanoparticles have the potential to deliver and release multiple cargos in an externally stimulated and site-specific manner. Such assemblies are currently structurally simplistic, comprising spherical capsules or lipid clusters. Given that form and function are intertwined, this lack of architectural complexity restricts the development of more sophisticated properties. To address this, we have devised an engineering strategy combining microfluidics and conjugation chemistry to synthesize nanosized liposomes with two discrete compartments, one within another, which we term concentrisomes. We can control the composition of each bilayer and tune both particle size and the dimensions between inner and outer membranes. We can specify the identity of encapsulated cargo within each compartment, and the biophysical features of inner and outer bilayers, allowing us to imbue each bilayer with different stimuli-responsive properties. We use these particles for multi-stage release of two payloads at defined time points, and as attolitre reactors for triggered in situ biochemical synthesis.

DOI: 10.1038/s41557-024-01584-z

Source: https://www.nature.com/articles/s41557-024-01584-z