德国马克斯·普朗克分子生物医学研究所Ivan Bedzhov和Hyun-Woo Jeong研究组，在单细胞水平分析胚胎休眠揭示了整合素-yap/Taz促生存信号的动态转录反应和激活。该项研究成果发表在2024年7月23日出版的《细胞-干细胞》上。

研究人员利用小鼠胚胎和单细胞RNA测序，在单细胞层面对胚胎休眠进行了分子水平的研究，揭示了胚胎处于休眠状态时的转录动态。

此外，研究还发现休眠的多能细胞依赖整合素受体来感知微环境，并通过Yap/Taz介导的前生存信号来维持其活力。

据悉，胚胎休眠是一种生殖适应行为，它使一些哺乳动物物种能够暂停持续的胚胎发育进程。在这种休眠状态下，胚胎利用特殊的调控机制来长时间保持其发育潜能。

附：英文原文

Title: Analyzing embryo dormancy at single-cell resolution reveals dynamic transcriptional responses and activation of integrin-Yap/Taz prosurvival signaling

Author: Rui Chen, Rui Fan, Fei Chen, Niraimathi Govindasamy, Heike Brinkmann, Martin Stehling, Ralf H. Adams, Hyun-Woo Jeong, Ivan Bedzhov

Issue&Volume: 2024-07-23

Abstract: Embryonic diapause is a reproductive adaptation that enables some mammalian species to halt the otherwise continuous pace of embryonic development. In this dormant state, the embryo exploits poorly understood regulatory mechanisms to preserve its developmental potential for prolonged periods of time. Here, using mouse embryos and single-cell RNA sequencing, we molecularly defined embryonic diapause at single-cell resolution, revealing transcriptional dynamics while the embryo seemingly resides in a state of suspended animation. Additionally, we found that the dormant pluripotent cells rely on integrin receptors to sense their microenvironment and preserve their viability via Yap/Taz-mediated prosurvival signaling.

DOI: 10.1016/j.stem.2024.06.015

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(24)00250-9